mouse anti human cd11a mab  (ATCC)

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Rheumatoid arthritis synovial stromal cells inhibit apoptosis and up-regulate Bcl-xL expression by B cells in a CD49/CD29-CD106-dependent mechanism.

doi: 10.4049/jimmunol.164.2.1110

Figure Lengend Snippet: FIGURE 7. Stimulation by VLA-4 can induce Bcl-xL protein and mRNA in B cells. B cells (1 3 106) were cultured for 3 days with mAbs to CD11a/CD18 and/or CD29/CD49d in 24-well culture plates coated with rabbit anti-mouse IgG. Afterward, the expression of Bcl-2 and Bcl-xL pro- tein was assessed by Western blotting, and the percentages of viable and apoptotic cells were measured as described in Materials and Methods, and mRNA for Bcl-2, Bcl-xL, and G6PD were assessed by RT-PCR as de- scribed in Materials and Methods. The results of Southern blotting are shown in Expt. 1, and the expression of PCR products by ethidium bromide staining is shown in Expt. 2. One to three micrograms of cDNA was used for each amplification. The number of PCR cycles was modified to ensure that the PCR products obtained were from the linear phase of amplification. The cycle number of each PCR is shown in the figure. Representative data from two of five experiments with similar results are shown.

Article Snippet: Mouse IgG1 (MOPC) mAb, mouse anti-human IgM heavy chain (DA4.4) conjugated with biotin, mouse anti-human CD11a mAb (TS1/22), and mouse antihuman CD18 mAb (TS1/18) were prepared from hybridoma cell lines purchased from American Type Culture Collection (Manassas, VA).

Techniques: Cell Culture, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Southern Blot, Staining